A new MetOSite release is now available. This new version includes results obtained by the group of Dr. Lionel Tarrago at Aix-Marseille University. A summary of this exciting work (Appl Environ Microbiol. 2024;90(3):e0193123. doi: 10.1128/aem.01931-23) provided by the authors can be found below.
“In this study, researchers investigated how white-rot fungi, specifically
Pycnoporus cinnabarinus, utilize carbohydrate-active enzymes (CAZymes) and
reactive oxygen species (ROS), such as hydrogen peroxide (H2O2), to break
down lignocellulose in wood. While the role of CAZymes in lignocellulose
degradation is well-known, the impact of ROS on the oxidation of secreted
proteins was unclear. Using a redox proteomics approach, the study revealed
that white-rot fungi protect their secreted proteins by minimizing
methionine (Met) content and employing a catalase to counteract oxidation.
The analysis identified 49 oxidizable Met residues in secreted proteins,
particularly in CAZymes, shedding light on the intricate mechanisms employed
by these fungi in wood decay. Notably, certain Met residues, including those
in GH7 cellobiohydrolases, were oxidized up to 47%, suggesting crucial
functional roles. These findings enhance our understanding of how white-rot
fungi safeguard their secreted proteins during the degradation of
lignocellulose.”
ptm 